Identification, Expression, and Substrate Specificity of a Mammalian β-Carotene 15,15′-Dioxygenase*

@article{Redmond2001IdentificationEA,
  title={Identification, Expression, and Substrate Specificity of a Mammalian $\beta$-Carotene 15,15′-Dioxygenase*},
  author={T Michael Redmond and S. Gentleman and T. Duncan and S. Yu and B. Wiggert and E. Gantt and F. X. Cunningham},
  journal={The Journal of Biological Chemistry},
  year={2001},
  volume={276},
  pages={6560 - 6565}
}
We have identified from mouse the first mammalian β-carotene 15,15′-dioxygenase (β-CD), a crucial enzyme in development and metabolism that governs the de novo entry of vitamin A from plant-derived precursors. β-CD is related to the retinal pigment epithelium-expressed protein RPE65 and belongs to a diverse family that includes the plant 9-cis-epoxycarotenoid dioxygenase and bacterial lignostilbene dioxygenases. β-CD expression in Escherichia coli cells engineered to produce β-carotene led to… Expand
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