Identification, Cloning, and Expression of Pseudomonas aeruginosa Phosphorylcholine Phosphatase Gene

@article{Massimelli2004IdentificationCA,
  title={Identification, Cloning, and Expression of Pseudomonas aeruginosa Phosphorylcholine Phosphatase Gene},
  author={M. J. Massimelli and P. Beassoni and M. Forrellad and J. L. Barra and M. Garrido and C. Domenech and A. T. Lisa},
  journal={Current Microbiology},
  year={2004},
  volume={50},
  pages={251-256}
}
Pseudomonas aeruginosa phosphorylcholine phosphatase (PChP) is a periplasmic enzyme produced simultaneously with the hemolytic phospholipase C (PLc-H) when the bacteria are grown in the presence of choline, betaine, dimethylglycine or carnitine. Molecular analysis of the P. aeruginosa mutant JUF8-00, after Tn5-751 mutagenesis, revealed that the PA5292 gene in the P. aeruginosa PAO1 genome was responsible for the synthesis of PChP. The enzyme expressed in E. coli, rPChP-Ec, purified by a chitin… Expand
Phosphorylcholine Phosphatase: A Peculiar Enzyme of Pseudomonas aeruginosa
TLDR
Pseudomonas aeruginosa synthesizes phosphorylcholine phosphatase (PchP) when grown on choline, betaine, dimethylglycine or carnitine, and the regulation of choline metabolism and consequently PchP synthesis may reflect an adaptive response of P. aerug inosa to environmental conditions. Expand
Preparation and biophysical characterization of recombinant Pseudomonas aeruginosa phosphorylcholine phosphatase.
Pseudomonas aeruginosa infections constitute a widespread health problem with high economical and social impact, and the phosphorylcholine phosphatase (PchP) of this bacterium is a potential targetExpand
Choline catabolism, σ⁵⁴ factor and NtrC are required for the full expression of the Pseudomonas aeruginosa phosphorylcholine phosphatase gene.
TLDR
It is demonstrated that choline catabolism and NtrC (nitrogen regulatory protein) are necessary for the full expression of pchP and is partially dependent on σ(54) factor. Expand
GbdR Regulates Pseudomonas aeruginosa plcH and pchP Transcription in Response to Choline Catabolites
TLDR
It is suggested that pchP and other genes involved in phosphorylcholine catabolism are necessary to stimulate the GbdR-mediated positive feedback induction of plcH in PC-rich environments, such as the lung. Expand
Critical Active-Site Residues Identified by Site-Directed Mutagenesis in Pseudomonas aeruginosa Phosphorylcholine Phosphatase, A New Member of the Haloacid Dehalogenases Hydrolase Superfamily
TLDR
Pseudomonas aeruginosa phosphorylcholine phosphatase (PChP), the product of the PA5292 gene, is synthesized when the bacteria are grown with choline, betaine, dimethylglycine, or carnitine and contained three conserved motifs characteristic of the haloacid dehalogenases superfamily. Expand
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TLDR
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Using a molecular model and kinetic experiments in the presence of divalent cations to study the active site and catalysis of Pseudomonas aeruginosa phosphorylcholine phosphatase.
TLDR
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Global regulator Anr represses PlcH phospholipase activity in Pseudomonas aeruginosa when oxygen is limiting.
TLDR
The presence of a negative feedback loop in which Anr repressed plcH transcription was demonstrated and it was proposed that this regulation allowed for PlcH levels to be maintained in a way that promotes productive host-pathogen interactions. Expand
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TLDR
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TLDR
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