Identical linkage and cooperativity of oxygen and carbon monoxide binding to Octopus dofleini hemocyanin.

@article{Connelly1989IdenticalLA,
  title={Identical linkage and cooperativity of oxygen and carbon monoxide binding to Octopus dofleini hemocyanin.},
  author={Patrick R. Connelly and Stanley J. Gill and Karen I. Miller and Guo-Ping Zhou and Kensal E van Holde},
  journal={Biochemistry},
  year={1989},
  volume={28 4},
  pages={1835-43}
}
Employment of high-precision thin-layer methods has enabled detailed functional characterization of oxygen and carbon monoxide binding for (1) the fully assembled form with 70 binding sites and (2) the isolated chains with 7 binding sites of Octopus dofleini hemocyanin. The striking difference in the cooperativities of the two ligands for the assembled decamer is revealed through an examination of the binding capacities and the partition coefficient, determined as functions of the activities of… CONTINUE READING

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