Ice nucleating activity of Pseudomonas syringae and Erwinia herbicola

  title={Ice nucleating activity of Pseudomonas syringae and Erwinia herbicola},
  author={Lloyd M. Kozloff and Mary Anne Schofield and Murl Lute},
  journal={Journal of Bacteriology},
  pages={222 - 231}
Chemical and biological properties of the ice nucleating sites of Pseudomonas syringae, strain C-9, and Erwinia herbicola have been characterized. The ice nucleating activity (INA) for both bacteria was unchanged in buffers ranging from pH 5.0 to 9.2, suggesting that there were no essential groups for which a change in charge in this range was critical. The INA of both bacteria was also unaffected by the addition of metal chelating compounds. Borate compounds and certain lectins markedly… 

Phosphatidylinositol as a Component of the Ice Nucleating Site of Pseudomonas syringae and Erwinia herbiola

Plant lectins binding to inositol and a highly purified phosphatidylinositol-specific hydrolase inhibited or decreased the efficiency of the ice nucleating activity (INA) of both bacteria.

Phosphatidylinositol, a phospholipid of ice-nucleating bacteria

Results indicate that PI plays an important role in ice nucleation at warm temperatures and is a likely precursor or component of the class A structure.

The ice Nucleation Activity of Pseudomonas fluorescens Migula and its Inhibition by Various Chemicals

The ‘tube-freezing’ method showed that on excised corn leaves treated with 1989/6259 and 1990/6155, the bacterial INA decreased while the super-cooling was more pronounced, and a significantly higher percentage of treated plants survived at −2 and −3°C compared to inoculated plants.

Preparation of active cell-free ice nuclei from Pseudomonas syringae

  • L. PooleyT. Brown
  • Biology
    Proceedings of the Royal Society of London. Series B: Biological Sciences
  • 1990
A method for preparing cell-free type I ice nuclei from Pseudomonas syringae is described and up to 3.6% of the type I nuclei present in the starting culture could be obtained in cell- free form, a substantially higher recovery level than previously achieved.

Components of ice nucleation structures of bacteria

Nonprotein components attached to the known protein product of the inaZ gene of Pseudomonas syringae have been identified and shown to be necessary for the most efficient ice nucleation of

Enhancement of the expression of ice‐nucleation activity of Pseudomonas fluorescens MACK‐4 isolated from mackerel

: The growth of Pseudomonas fluorescens MACK-4 isolated from mackerel in dextrin and starch broths was faster than that grown in other broths. The highest ice-nucleating activity (INA) was observed

Identification of a Compound in Chamaecyparis taiwanensis Inhibiting the Ice-nucleating Activity of Pseudomonas fluorescens KUIN-1

Inactivation of the ice-nucleating activity of Pseudomonas fluorescens KUIN-1 by compounds in the leaves from coniferous trees were investigated, and the inactivated material was identified as hinokitiol based on UV-VIS, IR, and mass spectral data.

Release of cell-free ice nuclei by Erwinia herbicola

Ice-nucleating activity in these cell-free preparations was associated with outer membrane vesicles shed by cells and was sensitive to protein-modifying reagents.

Inhibition of bacterial ice nucleation by polyglycerol polymers.

Three separate classes of bacterial ice nucleation structures

Studies of the properties of the ice nucleation structure exposed on the surfaces of various bacteria such as Pseudomonas syringae, Erwinia herbicola, or various strains of Ice+ recombinant



Distribution of ice nucleation-active bacteria on plants in nature

A replica plating method for rapid quantitation of ice nucleation-active (INA) bacteria was developed and numbers of INA bacteria were large enough to suggest that plant surfaces may constitute a significant source of atmospheric ice nuclei.

Defective packing of an unusual DNA in a virulent Erwinia phage, Erh 1.

According to the distribution of DNA sizes in these particles, the variations in sedimentation behavior, and the flexibility of the head structure of most particles, it appears that the headructure is formed first and then the DNA is packed inefficiently into this head structure.

Ice Nucleation Induced by Pseudomonas syringae

Broth cultures of suspensions of Pseudomonas syringae isolated from decaying alder leaves (Alnus tenuifolia) were found to freeze at very warm (-1.8 to -3.8 C) temperatures. The initiation of

Purification of the glycoprotein lectin from the broad bean (Vicia faba) and a comparison of its properties with lectins of similar specificity.

Broad-bean lectin was compared with concanavalin A and the lectins from pea and lentil in an investigation of the inhibition of their action by a number of monosaccharides, methyl ethers of mono-hexanoic acid derivative of agarose and glycopeptides.

Antifreeze proteins from fish bloods.

Biogenic Ice Nuclei. Part II: Bacterial Sources

Abstract Transient appearance of ice nuclei active at temperatures of −2 to −5°C has been noted to accompany the natural decay of plant leaf materials. It was shown that the development of these

Zinc uptake and incorporation into proteins in T4D bacteriophage-infected Escherichia coli.

The marked effect of infection of E. coli with T4D 12- on both zinc uptake and zinc incorporation into protein supports the conclusion that T 4D gene 12 protein is a zinc metalloprotein.

Bacteria as Biogenic Sources of Freezing Nuclei

Bacteria which produce freezing nuclei active at temperatures warmer than −10°C have been isolated from leaves, water and snow and the nucleating activity appears to be associated with the cell wall or cell wall fragments of the bacteria.