Ice-free cryopreservation of mouse embryos at −196 °C by vitrification

@article{Rall1985IcefreeCO,
  title={Ice-free cryopreservation of mouse embryos at −196 °C by vitrification},
  author={W. Rall and G. Fahy},
  journal={Nature},
  year={1985},
  volume={313},
  pages={573-575}
}
  • W. Rall, G. Fahy
  • Published 1985
  • Biology
  • Nature
  • The failure of complex mammalian organs, such as the kidney, to function following freezing to low temperatures is thought to be due largely to mechanical disruption of the intercellular architecture by the formation of extracellular ice1–5. Classical approaches to the avoidance of ice formation through the imposition of ultra-rapid cooling and warming rates6–8 or by gradual depression of the equilibrium freezing point during cooling to −80 °C9–13 have not been adequate. An alternative… CONTINUE READING
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