Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line.

@article{Liang2012IcariinPB,
  title={Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line.},
  author={Wenna Liang and Mu-nan Lin and Xi-hai Li and Candong Li and Bizhen Gao and Huijuan Gan and Zhaoyang Yang and Xuejuan Lin and Linghong Liao and Min Yang},
  journal={International journal of molecular medicine},
  year={2012},
  volume={30 4},
  pages={
          889-95
        }
}
Icariin, the main active compound of the traditional Chinese medicine, Epimedium, is commonly used for the clinical treatment of osteoporosis. However, the precise molecular mechanism of the therapeutic effect of icariin has not been elucidated. The aim of this study was to examine the effect of icariin on cell viability, alkaline phosphatase (ALP) activity, the amount of calcified nodules, and to delineate the molecular mechanism of icariin-enhanced bone formation by investigating the… 
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The effects of ICA on osteoblast differentiation from at least three perspectives are investigated to obtain an understanding of its mechanism of action and elucidate the molecular mechanisms of this drug, which might provide an insight into the rationality and in-depth theoretical basis for the clinical application of I CA in preventing osteoporosis.

Icariin promotes the proliferation and differentiation of osteoblasts from the rat mandible by the Wnt/β‑catenin signalling pathway.

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The BMP signaling pathway enhances the osteoblastic differentiation of bone marrow mesenchymal stem cells in rats with osteoporosis

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In Vitro and In Vivo Osteogenesis Induced by Icariin and Bone Morphogenetic Protein-2: A Dynamic Observation

It is found that in vitro ICA or BMP-2 treatment is able to increase osteoblast proliferation, which was detected by 3-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT), and ICA at a concentration of 30 µg/ml had the strongest ability to promote cell proliferation.
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References

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