Icariin-mediated expression of cardiac genes and modulation of nitric oxide signaling pathway during differentiation of mouse embryonic stem cells into cardiomyocytes in vitro

@article{Zhu2006IcariinmediatedEO,
  title={Icariin-mediated expression of cardiac genes and modulation of nitric oxide signaling pathway during differentiation of mouse embryonic stem cells into cardiomyocytes in vitro},
  author={Danyan Zhu and Y. Lou},
  journal={Acta Pharmacologica Sinica},
  year={2006},
  volume={27},
  pages={311-320}
}
AbstractAim:To investigate effects of icariin on cardiac gene expression and the modulation of nitric oxide (NO) signal transduction during the differentiation of embryonic stem (ES) cells into cardiomyocytes in vitro.Methods:The expression levels of cardiac developmental-dependent genes were measured using reverse transcription-polymerase chain reaction (RT-PCR). The chronotropic responses of cardiomyocytes to β-adrenoceptor stimulation were determined. The levels of cAMP and cGMP in ES cells… Expand
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