ITS1 versus ITS2 as DNA metabarcodes for fungi.

  title={ITS1 versus ITS2 as DNA metabarcodes for fungi.},
  author={Rakel Blaalid and Surendra Kumar and R. Henrik Nilsson and Kessy Abarenkov and Paul M. Kirk and H{\aa}vard Kauserud},
  journal={Molecular ecology resources},
  volume={13 2},
The nuclear ribosomal Internal Transcribed Spacer ITS region is widely used as a DNA metabarcoding marker to characterize the diversity and composition of fungal communities. In amplicon pyrosequencing studies of fungal diversity, one of the spacers ITS1 or ITS2 of the ITS region is normally used. In this methodological study we evaluate the usability of… CONTINUE READING