ISOLATION AND PARTIAL CHARACTERIZATION OF LYMPHOCYTE SURFACE IMMUNOGLOBULINS

@article{Marchalonis1972ISOLATIONAP,
  title={ISOLATION AND PARTIAL CHARACTERIZATION OF LYMPHOCYTE SURFACE IMMUNOGLOBULINS},
  author={John Jacob Marchalonis and Robert E. Cone and John L. Atwell},
  journal={The Journal of Experimental Medicine},
  year={1972},
  volume={135},
  pages={956 - 971}
}
Immunoglobulins were isolated from the surfaces of lymphocytes from a variety of lymphocyte populations including murine and human thymus lymphocytes and murine spleen and thoracic duct lymphocytes. Cell surface proteins were labeled with iodide-(125)I by lactoperoxidase-catalyzed iodination, and recovered in solution either by solubilization in dissociating solvents or active metabolic release. Immunoglobulins were identified and isolated by immunological coprecipitation. The polypeptide chain… CONTINUE READING

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