IP-10 and Mig production by glomerular cells in human proliferative glomerulonephritis and regulation by nitric oxide.

Abstract

High levels of expression of mRNA and protein for the chemokines interferon-gamma (IFN-gamma)-inducible protein of 10 kD (IP-10) (CXCL10) and the monokine induced by IFN-gamma (Mig) (CXCL9) were observed, by using in situ hybridization and immunohistochemical analyses, in kidney biopsy specimens from patients with glomerulonephritis (GN), particularly those with membranoproliferative or crescentic GN, but not in normal kidneys. Double-immunostaining or combined in situ hybridization and immunohistochemical analyses for IP-10, Mig, and proliferating cell nuclear antigen (PCNA) or alpha-smooth muscle actin (alpha-SMA) revealed that IP-10 and Mig production by resident glomerular cells was a selective property of glomeruli in which mesangial cells demonstrated active proliferation. IP-10 and Mig mRNA and protein were also expressed by primary cultures of human mesangial cells and human visceral epithelial cells after stimulation with IFN- gamma or with IFN-gamma plus tumor necrosis factor-alpha (TNF-alpha) (which produced greater stimulation). The induction of IP-10 and Mig mRNA and protein expression by IFN-gamma plus TNF-alpha was strongly inhibited by nitric oxide (NO) donors, such as sodium nitroprusside or S-nitroso-N-acetylpenicillamine, but not by cGMP analogues. Electrophoretic mobility shift assays demonstrated that NO donors repressed IP-10 gene transcription induced by IFN-gamma plus TNF-alpha through the inhibition of NF-kappaB activation. These data demonstrate that resident glomerular cells in kidneys of patients with proliferative GN produce large amounts of IP-10 and Mig, which may play important pathogenic roles in this disease. These data also indicate that the production of IP-10 and Mig by human mesangial cells can be downregulated by NO donors through cGMP-independent inhibition of NF-kappaB activation.

0200400'04'06'08'10'12'14'16
Citations per Year

912 Citations

Semantic Scholar estimates that this publication has 912 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Romagnani2002IP10AM, title={IP-10 and Mig production by glomerular cells in human proliferative glomerulonephritis and regulation by nitric oxide.}, author={Paola Romagnani and Elena Lazzeri and Laura Lasagni and Carmelo Mavilia and Chiara Beltrame and Michela Francalanci and Mario Rotondi and Francesco Annunziato and Lucia Maurenzig and Lorenzo Cosmi and Grazia Galli and Maurizio Salvadori and Enrico Maggi and Mario Serio}, journal={Journal of the American Society of Nephrology : JASN}, year={2002}, volume={13 1}, pages={53-64} }