INHIBITION OF HEXOSE AND GLYCEROL UTILIZATION BY 2-DEOXY-D-GLUCOSE IN TRYPANOSOMA GAMBIENSE AND TRYPANOSOMA RHODESIENSE.

@article{Seed1965INHIBITIONOH,
  title={INHIBITION OF HEXOSE AND GLYCEROL UTILIZATION BY 2-DEOXY-D-GLUCOSE IN TRYPANOSOMA GAMBIENSE AND TRYPANOSOMA RHODESIENSE.},
  author={J. Seed and M. Baquero and J. Duda},
  journal={Experimental parasitology},
  year={1965},
  volume={16},
  pages={
          363-8
        }
}
Abstract It was shown that 2-deoxy-d-glucose (D-2-D) inhibited the fermentation of glucose, mannose, fructose, and glycerol by Trypanosoma gambiense . Evidence is presented that shows D-2-D inhibits metabolism at two sites at least within the Embden-Meyerhof-Parnas pathway. The inhibition of both hexose and glycerol utilization by D-2-D was reversed by the addition of glucose; however, inhibition of glycerol utilization was much more sensitive to reversal. A second analogue of glucose… Expand
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References

SHOWING 1-10 OF 11 REFERENCES
Evidence for hexose transport in acetone-dried yeast.
  • T. Scharff
  • Biology, Medicine
  • Archives of biochemistry and biophysics
  • 1961
TLDR
It is concluded that the lyophilization and acetone-extraction procedure leaves relatively intact a transfer mechanism in “dried” cells which are still capable of fermentation. Expand
METABOLISM OF SCHIZOTRYPANUM CRUZI CHAGAS. II. GALACTOSE UTILIZATION.
TLDR
Kinetic analysis of galactose utilization indicates that an active process and a diffusion component are involved in the utilization of this sugar by Schizotrypanum cruzi, which appears to set S. cruzi apart from other trypanosomes. Expand
The effect of 2-desoxy-D-glucose on glycolysis and respiration of tumor and normal tissues.
TLDR
2DG in vivo might have a selective inhibitory effect on tumor metabolism, since inhibition was reversed by increased glucose in the case of tumor and brain, and 2DG did not significantly inhibit the endogenous respiration in any of the tissues studied. Expand
A tentative mechanism for the anaerobic transport of glucose, fructose and mannose in yeast.
TLDR
Under anaerobic conditions, glucose, fructose, and mannose were added separately or in pairs to yeast suspensions to postulated and tested a carrier-mediated diffusion process similar to that for sugars in human erythrocyytes. Expand
The chemical composition of trypanosomes. I. Protein, amino acid and sugar analysis.
TLDR
Intracellular free amino acids were demonstrable in all organisms at a level of approximately 2.5% (w/w) of cellular protein; T. lewisi was exceptional in showing a much lower level; the free amino acid pattern was characterized by a high content of alanine. Expand
The catabolism of glucose by strains of Trypanosoma rhodesiense.
TLDR
A chronology of key events leading up to and including the invention of the biol in 1953:. Expand
Characterization of antigens isolated from Trypanosoma rhodesiense.
TLDR
Two blood trypanosome antigens (PR and AG) have been isolated from the serum of infected rats and appear to be proteins, which suggested that there are probably numerous antIGens common to different strains of T. rhodesiense which have not been detected by this technique. Expand
Protein measurement with the Folin phenol reagent.
TLDR
Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein. Expand
The Uptake and Fate of Radioactive Elements in Trypanosoma Equiperdum
T. equiperdum was supplied by the Walter Reed Army Institute of Research, Division of Veterinary Medicine. The strain was maintained by rat passage every two days. The organisms were separated fromExpand
...
1
2
...