IMS-free DNA extraction for the PCR-based quantification of Cryptosporidium parvum and Giardia lamblia in surface and waste water.

@article{Anceno2007IMSfreeDE,
  title={IMS-free DNA extraction for the PCR-based quantification of Cryptosporidium parvum and Giardia lamblia in surface and waste water.},
  author={Alfredo J. Anceno and Hiroyuki Katayama and Eric R Houpt and Porntip Chavalitshewinkoon-Petmitr and Buyan Chuluun and Oleg V. Shipin},
  journal={International journal of environmental health research},
  year={2007},
  volume={17 4},
  pages={297-310}
}
Extremely limited knowledge exists on the occurrence of protozoan pathogens in surface and waste water in the developing world. The article addresses one of the major reasons for this: prohibitively costly immunomagnetic separation (IMS) and commercial DNA extraction kits are required for the pathogen detection. As the presence of inhibitory substances… CONTINUE READING