IMMUNOGLOBULIN PURIFICATION BY AFFINITY CHROMATOGRAPHY USING PROTEIN A MIMETIC LIGANDS PREPARED BY COMBINATORIAL CHEMICAL SYNTHESIS

@article{Kabir2002IMMUNOGLOBULINPB,
  title={IMMUNOGLOBULIN PURIFICATION BY AFFINITY CHROMATOGRAPHY USING PROTEIN A MIMETIC LIGANDS PREPARED BY COMBINATORIAL CHEMICAL SYNTHESIS},
  author={Shahjahan Kabir},
  journal={Immunological Investigations},
  year={2002},
  volume={31},
  pages={263 - 278}
}
  • S. Kabir
  • Published 1 January 2002
  • Medicine, Biology
  • Immunological Investigations
Affinity chromatography using protein A from Staphylococcus aureus as the ligand has been widely used for the isolation of immunoglobulin G (IgG) from various species. Since ligand leakage from the affinity support can occur, time consuming analytical controls are required to detect the presence of contaminants associated with the isolated IgG prior to its use for therapeutic purpose in humans. Besides, protein A is an expensive bacterial product, whose isolation involves complex and labor… 
Purification of antibodies using the synthetic affinity ligand absorbent MAbsorbent A2P
TLDR
A protocol for the purification of polyclonal antibodies from ovine serum using the synthetic protein A absorbent MAbsorbent A2P is described, showing robustness, relatively low cost, ease of sanitization and, in principle, lack of biological contamination.
Affinity purification of IgG monoclonal antibodies using the D-PAM synthetic ligand: chromatographic comparison with protein A and thermodynamic investigation of the D-PAM/IgG interaction.
TLDR
D-PAM affinity columns, prepared by immobilizing the all-D peptide on the commercially available support Emphaze, were able to capture monoclonal antibodies in a single chromatographic step, with a recovery yield and purity degree above 90% and full recovery of antibody activity.
One-step affinity purification of recombinant urokinase-type plasminogen activator receptor using a synthetic peptide developed by combinatorial chemistry.
TLDR
This study compares the affinity purification of a soluble, recombinant uPAR using the monoclonal antibody R2 or the peptide AE152 immobilized on Sepharose and finds that the two affinity ligands perform equally well in purifying uPAR.
Separation of antigens and antibodies by immunoaffinity chromatography
TLDR
It is anticipated that the improvements of IAC will have impact not only on large-scale production of antibodies but also on the generation of new affinity-based methods for the increasing number of proteins and antibody derivatives available by protein engineering and the proteomics revolution.
Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides
TLDR
In this review, recent developments of Fc-binding peptides are presented and their binding characteristics and diverse applications are discussed.
Screening of peptide ligands that bind to the Fc region of IgG using peptide array and its application to affinity purification of antibody
Abstract Small peptides have attracted great interest for affinity purification since they are more stable, less immunogenic, and less expensive compared to protein ligands. In this study, screening
Affinity chromatography matures as bioinformatic and combinatorial tools develop.
  • Y. Clonis
  • Chemistry, Medicine
    Journal of chromatography. A
  • 2006
TLDR
Affinity chromatography has the reputation of a more expensive and less robust than other types of liquid chromatography, but is designed to offer high specificity, being able to safely guide protein manufactures to successfully cope with the aforementioned challenges.
Affinity chromatography as a tool for antibody purification.
TLDR
The approaches used for antibody purification are critically examined with the aim of providing the reader with the principles and practical insights required to understand the intricacies of the procedures.
Aptamer-Based Affinity Chromatography for Protein Extraction and Purification.
TLDR
This chapter first discusses the context of the affinity chromatography with aptamer ligands, with the adaptation of SELEX, the chemical modifications of aptamers to comply with the covalent coupling and the separation process are extensively presented.
Affinity Chromatographic Purification of Antibodies
Abstract Affinity chromatographic methods for purifying antibodies are reviewed. Topics reviewed include (a) the matrices used in the preparation of the affinity supports; (b) the chemistries
...
1
2
3
4
...

References

SHOWING 1-10 OF 58 REFERENCES
Affinity purification of immunoglobulin M using a novel synthetic ligand.
TLDR
Antibody purity after affinity purification was very high, close to 85-95%, as determined by densitometric scanning of sodium dodecyl sulfate-polyacrylamide gels of purified fractions, and by gel permeation analysis.
Protein a mimetic peptide ligand for affinity purification of antibodies
TLDR
The tetrameric tripeptide identified after three screening cycles was produced in larger amounts and then immobilized in high yield on preactivated solid support for the preparation of affinity columns, which proved useful for a very convenient one‐step purification of antibodies directly from crude sera.
Affinity purification of mouse monoclonal IgE using a protein A mimetic ligand (TG19318) immobilized on solid supports
TLDR
TG19318 affinity columns proved useful for a very convenient one‐step purification of IgE directly from crude ascites, as determined by immunoassays on antigen‐coated plates, and up to 5 mg of IgEs could be purified on a 1 ml column in a single run.
Affinity purification of immunoglobulins from chicken egg yolk using a new synthetic ligand.
TLDR
The derivatized matrix of TG19318/Emphaze was found to be very stable, in terms of ligand leakage and maintenance of IgY binding capacity, under conditions of normal column usage, cleaning and storage.
A preliminary study for isolation of catalytic antibodies by histidine ligand affinity chromatography as an alternative to conventional protein A/G methods
TLDR
Catalytic autoimmune antibodies from the sera of lupus patients were purified using histidyl-aminohexyl-Sepharose gel and compared with the antibodies purified with protein A and protein G affinity chromatography, finding histidine affinity offer a superior method for isolating autoimmune catalytic antibodies.
A synthetic ligand for IgA affinity purification
TLDR
TG19318, a synthetic ligand deduced from the screening of combinatorial libraries, displays specific and selective recognition properties for immunoglobulins of the G class and can be used conveniently for affinity chromatography purification of monoclonal and polyclonal antibodies.
Immunoglobulin specificity of TG19318: a novel synthetic ligand for antibody affinity purification
TLDR
Validation of antibody affinity purification processes for therapeutic use is going to be simplified by the use of TG19318, which could reduce considerably the presence of biological contaminants in the purified preparation, a very recurrent problem when using recombinant or extractive biomolecules as affinity ligands.
Comparison of immunoglobulin binding capacities and ligand leakage using eight different protein A affinity chromatography matrices.
TLDR
Amongst the gels with IgG 3 capacities greater than 10 mg/ml, the least contamination with protein A was observed in the IgG3 fractions from immobilized rProtein A and Protein A-Sepharose CL-4B (Fermentech).
A one-step purification of membrane proteins using a high efficiency immunomatrix.
A method is described by which an immunoaffinity matrix was constructed by binding antibody directly or indirectly to protein A-Sepharose 4B followed by cross-linking of the complex with dimethyl
A strategy for the generation of biomimetic ligands for affinity chromatography. Combinatorial synthesis and biological evaluation of an IgG binding ligand
TLDR
An IgG‐binding ligand library comprising 88 adsorbents based on a known lead compound was generated on an agarose solid phase and it was found that ligands comprising 3‐aminophenol and an aminonaphthol moiety substiuted on a triazine nucleus generally performed better than other ligands in the library.
...
1
2
3
4
5
...