IM-TORNADO: A Tool for Comparison of 16S Reads from Paired-End Libraries

  title={IM-TORNADO: A Tool for Comparison of 16S Reads from Paired-End Libraries},
  author={Patricio R Jeraldo and Krishna R. Kalari and Xianfeng Chen and Jaysheel D. Bhavsar and Ashutosh K Mangalam and Bryan White and Heidi Nelson and Jean-Pierre A. Kocher and Nicholas Wai Kit Chia},
  booktitle={PloS one},
MOTIVATION 16S rDNA hypervariable tag sequencing has become the de facto method for accessing microbial diversity. Illumina paired-end sequencing, which produces two separate reads for each DNA fragment, has become the platform of choice for this application. However, when the two reads do not overlap, existing computational pipelines analyze data from read separately and underutilize the information contained in the paired-end reads. RESULTS We created a workflow known as Illinois Mayo Taxon… CONTINUE READING
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