IL-27 enhances Leishmania amazonensis infection via ds-RNA dependent kinase (PKR) and IL-10 signaling.

Abstract

The protozoan parasite Leishmania infects and replicates in macrophages, causing a spectrum of diseases in the human host, varying from cutaneous to visceral clinical forms. It is known that cytokines modulate the immunological response against Leishmania and are relevant for infection resolution. Here, we report that Interleukin (IL)-27 increases Leishmania amazonensis replication in human and murine macrophages and that the blockage of the IL-10 receptor on the surface of infected cells abolished the IL-27-mediated enhancement of Leishmania growth. IL-27 induced the activation/phosphorylation of protein kinase R (PKR) in macrophages, and PKR blockage or PKR gene deletion abrogated the enhancement of the parasite growth driven by IL-27, as well as the L. amazonensis-induced macrophage production of IL-27. We also observed that L. amazonensis-induced expression of IL-27 depends on type I interferon signaling and the engagement of Toll-like receptor 2. Treatment of Leishmania-infected mice with IL-27 increased lesion size and parasite loads in the footpad and lymph nodes of infected animals, indicating that this cytokine exerts a local and a systemic effect on parasite growth and propagation. In conclusion, we show that IL-27 is a L. amazonensis-enhancing factor and that the PKR/IFN1 axis and IL-10 are critical mediators of this IL-27 induced effect.

DOI: 10.1016/j.imbio.2014.11.006
02040201520162017
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@article{BarretodeSouza2015IL27EL, title={IL-27 enhances Leishmania amazonensis infection via ds-RNA dependent kinase (PKR) and IL-10 signaling.}, author={Victor Barreto-de-Souza and Pedro Leite Ferreira and {\'A}islan de Carvalho Vivarini and Teresa Cristina Calegari-Silva and Deivid Costa Soares and Eduardo G. Regis and Renata de Meirelles Santos Pereira and Arist{\'o}bolo Mendes da Silva and Elvira Maria Breier Saraiva and Ulisses Gazos Lopes and Dumith Chequer Bou-Habib}, journal={Immunobiology}, year={2015}, volume={220 4}, pages={437-44} }