IDENTIFICATION OF EUROPEAN DIPLOZOIDS (MONOGENEA, DIPLOZOINAE) BY RESTRICTION DIGESTION OF THE RIBOSOMAL RNA INTERNAL TRANSCRIBED SPACER

@inproceedings{Matjusov2004IDENTIFICATIONOE,
  title={IDENTIFICATION OF EUROPEAN DIPLOZOIDS (MONOGENEA, DIPLOZOINAE) BY RESTRICTION DIGESTION OF THE RIBOSOMAL RNA INTERNAL TRANSCRIBED SPACER},
  author={I. Matějusov{\'a} and B. Koubkov{\'a} and C. O. Cunningham},
  booktitle={The Journal of parasitology},
  year={2004}
}
The second internal transcribed spacer (ITS2) of the ribosomal RNA genes of Diplozoon paradoxum and Paradiplozoon nagibinae were amplified and sequenced. The polymerase chain reaction product of D. paradoxum was bigger (840 bp) than that of P. nagibinae (820 bp). There was no intraspecific variability recorded in sequences from either species. Sequence comparisons and ITS2 restriction fragment length polymorphism (RFLP) pattern of 8 European diplozoid species aimed to resolve their… Expand
Three new Diplozoidae mitogenomes expose unusual compositional biases within the Monogenea class: implications for phylogenetic studies
TLDR
This study sequenced and characterized mitogenomes belonging to three Diplozoidae family species, and conducted comparative genomic and phylogenomic analyses for the entire Monogenea class, showing that nad2, atp6 and nad6 (fast-evolving) would be better candidates than cox1 (slow-evolved) for species identification and population genetics studies in Diplozooidae. Expand
Morphological and Molecular Characterization of Diplozoon kashmirensis;D. aegyptensis and D. guptai Collected from Fishes of Kashmir Valley-India
TLDR
The study reports the results of molecular characterization of the Internal Transcribed Spacer (ITS) of ribosomal DNA of 3 Monogenean species using polymerase chain reaction (PCR), nucleotide sequencing and construction of phylogenetic trees from different fish hosts of Kashmir revealing that there are intraspecific differences in their base pair lengths. Expand
Comparative molecular characterization of three Diplozoon species from fishes of Kashmir Valley.
TLDR
The present study shows that the size of the amplified product is 873 bp long for D. kashmirensis and D. guptai revealing that there are intraspecific differences in their base pair lengths, and Guanine and Cytocine (G+C) content of three Diplozoon species was found nearly constant for three species, contributing to physical attributes of RNA structures. Expand
Paradiplozoon vaalense n. sp. (Monogenea: Diplozoidae) from the gills of moggel, Labeo umbratus (Smith, 1841), in the Vaal River System, South Africa.
TLDR
Data clearly indicate that diplozoids collected from moggel represent a new, distinct taxon of Paradiplozoon Akhmerov, 1974 and are described here as ParadiPlozoon vaalense n. Expand
Paradiplozoon skrjabini ( Monogenea , Diplozoidae ) , an Ectoparasite on the Gills of Freshwater Fishes ( Cyprinidae , Leuciscinae ) of Japan and Primorsky Region , Russia : a Morphological and Molecular Study
Monogenean specimens of Paradiplozoon Akhmerov, 1974 (Diplozoidae) were found on the gills of Tribolodon hakonensis (Günther, 1877), Tribolodon sachalinensis (Nikolskii, 1889), Phoxinus steindachneriExpand
The molecular and morphometrical description of a new diplozoid species from the gills of the Garra rufa (Heckel, 1843) (Cyprinidae) from Turkey—including a commentary on taxonomic division of Diplozoidae
TLDR
This is the first diplozoid species to be described from G. rufa. Expand
Redescription of the genus Afrodiplozoon Khotenovski, 1981 and its only known species Afrodiplozoon polycotyleus (Paperna, 1973) (Monogenea: Diplozoidae) using a combined multidisciplinary approach.
TLDR
Molecular characterization of the second internal transcribed spacer (ITS2) rDNA and phylogenetic analysis revealed the position of Afrodiplozoon polycotyleus as a sister taxon to Paradiplozoons krugerense and ParadiPlozoon bingolenisis, distant from other African representatives of the genus Paradi plozoon. Expand
Cytogenetics of Eudiplozoon nipponicum (Monogenea, Diplozoidae): Karyotype, spermatocyte division and 18S rDNA location.
TLDR
The results of the cytogenetic analysis are consistent with the hypothesis that E. nipponicum is more basal member of the family Diplozoidae and compared with studies on the phylogenetic relationships of diplozoids which applied a combination of molecular methods and classical morphological characterization. Expand
Morphological and molecular studies of Eudiplozoon nipponicum (Goto, 1891) and Eudiplozoon kamegaii sp. n. (Monogenea; Diplozoidae).
TLDR
Results of a taxonomic re-examination of E. nipponicum using genetic analysis and morphological comparisons from different host species from a single water system indicate that diplozoids from C. carpio and species of Carassius belong to different species. Expand
Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling
TLDR
Two high-throughput sequencing techniques, mass spectrometry analysis, and comprehensive bioinformatics approach are integrated in order to arrive at the first comprehensive description of monogenean transcriptome and secretome of E. nipponicum. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 20 REFERENCES
Genetic characterization of six species of diplozoids (Monogenea; Diplozoidae)
TLDR
The second internal transcribed spacer (ITS2) of the ribosomal RNA gene array of 6 species of diplozoids, E. nipponicum was the most genetically distinct species, whereas P. bliccae and P. sapae were the species most closely related. Expand
Species variation within the internal transcribed spacer (ITS) region of Gyrodactylus (Monogenea:Gyrodactylidae) ribosomal RNA genes.
TLDR
The ribosomal internal transcribed spacer (ITS) region from individual Gyrodactylus specimens was amplified by polymerase chain reaction (PCR) and enables rapid and clear differentiation of G. salaris, G. derjavini, and G. truttae. Expand
Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei).
TLDR
Results confirm the utility of both variable region V4 and the ITS as molecular markers for Gyrodactylus species and minor differences in the sequences between Western and Eastern European populations were detected. Expand
Paradiplozoon homoion Bychowsky & Nagibina, 1959 versus P. gracile Reichenbach-Klinke, 1961 (Monogenea): two species or phenotypic plasticity?
TLDR
The length of the median plate of the attachment clamps may be a useful character for species identification of diplozoids and is suggested for considering P. gracile as a species inquirenda. Expand
Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences.
TLDR
PCR-based restriction fragment length polymorphism and single-strand conformation polymorphism methods were established for the unequivocal delineation of the three species and should provide valuable tools for studying the life-cycle, transmission pattern(s), population structure and diagnosis of anisakiasis in humans and animals. Expand
Caractérisation moléculaire des populations de Diplozoidae sur cinq espèces de Cyprinidae: nouvelles données sur la spécificité parasitaire
TLDR
For the first time in the Diplozoidae, the problems of species definition and of host specificity are examined using molecular tools and the comparison between these two molecular phylogenies seems to exhibit the phenomenon of cospeciation. Expand
Global Spread of the Eel Parasite Gyrodactylus Anguillae (Monogenea)
TLDR
Gyrodactylus anguillae probably originated in Europe, and it is predicted that the worm has a high potential to invade eel populations in East Africa, Southeast Asia, and New Zealand. Expand
Phylogenetics of the Monogenea--evidence from a medley of molecules.
TLDR
Deep branches of the Heteronchoinea were similar for both classes of data but also showed that the interrelationships of the mazocraeidean families are labile and susceptible to sampling. Expand
Identification of Gyrodactylus (Monogenea) species parasitizing salmonid fish using DNA probes
TLDR
Oligonucleolides GsV4B, GdV4 and GtV4, complementary to sequences from Gyrodactylus Salaris, G. derjavini and G. truttae, respectively, were designed following examination of the sequence of the small subunit ribosomal RNA (srRNA) gene from each species, providing an objective, sensitive and specific method suitable for routine diagnostic use in identifying GyrodACTylus species. Expand
ITS-1 ribosomal DNA sequence variants are maintained in different species and strains of Echinococcus.
TLDR
Investigation of sequence heterogeneity in the first internal transcribed spacer (ITS-1) of ribosomal DNA within and among species and strains of Echinococcus provided evidence that at least two turnover mechanisms, namely slippage and unequal crossing over/transposition, have led to the divergence and maintenance of sequence variants in E Chinococcusspecies and strains. Expand
...
1
2
...