• DOI:10.1080/10826076.2010.532716
• Corpus ID: 97500340

IDENTIFICATION AND DETERMINATION OF THE MAJOR CONSTITUENTS IN AN ANTIANGIOGENESIS HERBAL FORMULA, QING-LUO-FANG, BY HPLC—DAD—ESI/MS

@article{Yang2010IDENTIFICATIONAD,
  title={IDENTIFICATION AND DETERMINATION OF THE MAJOR CONSTITUENTS IN AN ANTIANGIOGENESIS HERBAL FORMULA, QING-LUO-FANG, BY HPLC—DAD—ESI/MS},
  author={Shuai Yang and Kai-tong Li and Yi-xuan Zhang and Shao-Hui Li and Yue Shi},
  journal={Journal of Liquid Chromatography \& Related Technologies},
  year={2010},
  volume={33},
  pages={1842 - 1853}
}

• Shuai Yang, Kai-tong Li, +2 authors Yue Shi
• Published 30 November 2010
• Chemistry
• Journal of Liquid Chromatography & Related Technologies

A high-performance liquid chromatography—diode array UV detection—electrospray ionization tandem mass spectrometry (HPLC—DAD—ESI-MS) method was established for the simultaneous qualitative and quantitative analysis of the major bioactive constituents in Qing-Luo-Fang (QLF), an anti-angiogenesis herbal formula. The separation was performed on a Phenomenex Gemini C18 column using gradient elution. The mobile phase A was ammonium acetate (10 mM) in water; and mobile phase B was methanol-acetonitrile (1:3, v/v) containing 10 mM ammonium acetate and 0.2% ammonia. Twelve compounds were identified by online ESI-MS, and eight of which were quantitated by HPLC—DAD. The eight compounds are major alkaloid compounds present in QLF, including magnoflorine, oxymatrine, oxysophocarpine, sinomenine, sophocarpine, matrine, palmatine, and berberine. The method was validated and the linearities (r2 > 0.999) and recoveries (ranged from 95.24 to105.50%) were acceptable. The limits of detection (LOD) and the limits of quantitation (LOQ) of these alkaloids ranged from 0.63 to 6.70 ng/mL and from 2.01 to 20.69 ng/mL, respectively. The intra- and inter-day precision was less than 3% and the accuracy ranged from 97.4% to 102.7%. These results prove that this established method can be used to identify the structure of active constituents responsible for the pharmacological effects of QLF and to control the quality of QLF.