ICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T-cell priming.

@article{Segura2005ICAM1OE,
  title={ICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T-cell priming.},
  author={Elodie Segura and Carole Nicco and B{\'e}rang{\`e}re Lombard and Philippe Véron and Graça Raposo and Fr{\'e}d{\'e}ric Batteux and S{\'e}bastian Amigorena and Clotilde Th{\'e}ry},
  journal={Blood},
  year={2005},
  volume={106 1},
  pages={
          216-23
        }
}
Exosomes are secreted vesicles formed in late endocytic compartments. Immature dendritic cells (DCs) secrete exosomes, which transfer functional major histocompatibility complex (MHC)-peptide complexes to other DCs. Since immature and mature DCs induce different functional T-cell responses (ie, tolerance versus priming), we asked whether DC maturation also influenced the priming abilities of their exosomes. We show that exosomes secreted by lipopolysaccharide (LPS)-treated mature DCs are 50- to… 

Figures and Tables from this paper

Activated T cells recruit exosomes secreted by dendritic cells via LFA-1.

TLDR
It is shown that T cells can recruit major histocompatibility complex class II-containing DC exosomes secreted in the extracellular milieu during cognate DC-T-cell interactions and that these exosome transfers are targeted to T cells activated in that microenvironment.

CD8+ Dendritic Cells Use LFA-1 to Capture MHC-Peptide Complexes from Exosomes In Vivo1

TLDR
It is shown that exosomes can be presented by mouse DCs without the need for internalization and processing, and a new role for LFA-1 is proposed, as a receptor for exosome capture to favor Ag transfer between DCs in vivo.

Exosomes As a Short-Range Mechanism to Spread Alloantigen between Dendritic Cells during T Cell Allorecognition1

TLDR
The results suggest that exchange of exosomes between DCs in lymphoid organs might constitute a potential mechanism by which passenger leukocytes transfer alloantigens to recipient’s APCs and amplify generation of donor-reactive T cells following transplantation.

Mechanism of transfer of functional microRNAs between mouse dendritic cells via exosomes.

TLDR
It is demonstrated that DCs release exosomes with different miRNAs depending on the maturation of the DCs, and exosome-shuttle miRNAAs are functional, because they repress target mRNAs of acceptor DCs.

Qualitative differences in T‐cell activation by dendritic cell‐derived extracellular vesicle subtypes

TLDR
It is shown that large EVs (lEVs) released by human DCs are as efficient as small EVs (sEVs), including exosomes, to induce CD4+ T‐cell activation in vitro.

Exosomes : immunomodulators in cancer and therapy

TLDR
New insights are given into how to design vesicle-based cancer vaccines and provide new opportunities for the use of allogeneic DC-derived exosomes in patients, as well as investigating whether MHC complexes on exosome are needed to induce an anti-tumour immune response.

EXOSOMES - NANO-VESICLES IN IMMUNE REGULATION

TLDR
The work presented in this thesis has increased the understanding of the presence of human exosomes in vivo, and addressed how differentExosomes found in vivo may exert distinct immunomodulatory effects.

MHC Class II+ Exosomes in Plasma Suppress Inflammation in an Antigen-Specific and Fas Ligand/Fas-Dependent Manner1

TLDR
Results suggest that exosomes in the plasma, produced by MHC class II+ and CD11b+ cells, have the ability to suppress the immune response in an Ag-specific manner in part through a Fas/FasL-dependent manner.

No Significant CTL Cross-Priming by Dendritic Cell-Derived Exosomes during Murine Lymphocytic Choriomeningitis Virus Infection1

TLDR
Data support that exosomes can transfer Ags to dendritic cells (DC), and, interestingly, that these DC can subsequently induce T cell priming or tolerance, and whether this concept can be expanded to antiviral immunity is investigated.
...

References

SHOWING 1-10 OF 44 REFERENCES

Indirect activation of naïve CD4+ T cells by dendritic cell–derived exosomes

TLDR
It is found that injection of antigen- or peptide-bearing exosomes induced antigen-specific naïve CD4+ T cell activation in vivo and may increase the number of DCs bearing a particular peptide, thus amplifying the initiation of primary adaptive immune responses.

Exosomes bearing HLA-DR1 molecules need dendritic cells to efficiently stimulate specific T cells.

TLDR
Exosomes bearing MHC class II complexes must be taken up by professional APC for efficient T cell activation, and the incubation of free exosomes with DC resulted in the highly efficient stimulation of specific T cells.

Exosomes as Potent Cell-Free Peptide-Based Vaccine. I. Dendritic Cell-Derived Exosomes Transfer Functional MHC Class I/Peptide Complexes to Dendritic Cells 1

TLDR
Exosomes require nature’s adjuvants to efficiently promote the differentiation of melanoma-specific effector T lymphocytes producing IFN-γ (Tc1) effector lymphocytes in HLA-A2 transgenic mice (HHD2).

Endocytosis, intracellular sorting, and processing of exosomes by dendritic cells.

TLDR
It is demonstrated that exosomes also are internalized and processed by immature DCs for presentation to CD4(+) T cells, implying that exOSomes present in circulation or extracellular fluids constitute an alternative source of self- or allopeptides for DCs during maintenance of peripheral tolerance or initiation of the indirect pathway of allorecognition in transplantation.

Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid

TLDR
The results demonstrate that exosomes are present in the lung, and since they contain both major histocompatibility complex and co-stimulatory molecules it is likely that they are derived from antigen presenting cells and might have a regulatory role in local immune defence.

Exosomes as a Tumor Vaccine: Enhancing Potency Through Direct Loading of Antigenic Peptides

TLDR
It is demonstrated that MHC-I on purified exosomes can be directly loaded with peptide at much greater levels than indirect loading, and this increase in peptide binding greatly enhanced exosome potency, allowing us to further study the biologic activity ofExosomes in vitro.

Exosomes as Potent Cell-Free Peptide-Based Vaccine. II. Exosomes in CpG Adjuvants Efficiently Prime Naive Tc1 Lymphocytes Leading to Tumor Rejection 1

TLDR
Exosome immunogenicity across species allowed to verify the efficacy of good manufactory procedures-manufactured human exosomes admixed with CpG oligonucleotides in prophylactic and therapeutic settings of melanoma in HLA-A2 transgenic mice.

Presentation of donor major histocompatibility complex antigens by bone marrow dendritic cell-derived exosomes modulates allograft rejection1

TLDR
The authors demonstrate an effect of allogeneic exosome on the modulation of immune responses in vivo, suggesting that, like donor cells, exosomes can stimulate or regulate antigen-specific immune responses.

Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell derived exosomes

TLDR
Dendritic cells are shown to secrete antigen presenting vesicles, called exosomes, which express functional Major Histocompatibility Complex class I and class II, and T-cell costimulatory molecules, which prime specific cytotoxic T lymphocytes in vivo.