I5M: 3D widefield light microscopy with better than 100 nm axial resolution.

@article{Gustafsson1999I5M3W,
  title={I5M: 3D widefield light microscopy with better than 100 nm axial resolution.},
  author={M G Gustafsson and David A. Agard and John Sedat},
  journal={Journal of microscopy},
  year={1999},
  volume={195 Pt 1},
  pages={10-6}
}
Sevenfold improved axial resolution has been achieved in three-dimensional widefield fluorescence microscopy, using a novel interferometric technique in which the sample is observed and/or illuminated from both sides simultaneously using two opposing objective lenses. Separate interference effects in the excitation light and the emitted light give access to higher resolution axial information about the sample than can be reached by conventional widefield or confocal microscopes. Here we report… CONTINUE READING

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