I-SceI-mediated double-strand DNA breaks stimulate efficient gene targeting in the industrial fungus Trichoderma reesei

@article{Ouedraogo2015ISceImediatedDD,
  title={I-SceI-mediated double-strand DNA breaks stimulate efficient gene targeting in the industrial fungus Trichoderma reesei},
  author={Jean Paul Ouedraogo and Mark Arentshorst and I. N. Nikolaev and Sharief Barends and Arthur F. J. Ram},
  journal={Applied Microbiology and Biotechnology},
  year={2015},
  volume={99},
  pages={10083-10095}
}
Targeted integration of expression cassettes for enzyme production in industrial microorganisms is desirable especially when enzyme variants are screened for improved enzymatic properties. However, currently used methods for targeted integration are inefficient and result in low transformation frequencies. In this study, we expressed the Saccharomyces… CONTINUE READING