I-DIRT, a general method for distinguishing between specific and nonspecific protein interactions.

@article{Tackett2005IDIRTAG,
  title={I-DIRT, a general method for distinguishing between specific and nonspecific protein interactions.},
  author={Alan J. Tackett and Jeffrey A Degrasse and Matthew D. Sekedat and Marlene Oeffinger and Michael P. Rout and Brian T Chait},
  journal={Journal of proteome research},
  year={2005},
  volume={4 5},
  pages={1752-6}
}
Isolation of protein complexes via affinity-tagged proteins provides a powerful tool for studying biological systems, but the technique is often compromised by co-enrichment of nonspecifically interacting proteins. We describe a new technique (I-DIRT) that distinguishes contaminants from bona fide interactors in immunopurifications, overcoming this most challenging problem in defining protein complexes. I-DIRT will be of broad value for studying protein complexes in biological systems that can… CONTINUE READING
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Exploring Cells with A Centrifuge

C. de Duve
Nobel Lectures in Physiology 1971 - • 1980

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