Hypoxic alterations in neonatal neurons: an acridine orange fluorochromic study of nucleic acids.

@article{Sarnat1987HypoxicAI,
  title={Hypoxic alterations in neonatal neurons: an acridine orange fluorochromic study of nucleic acids.},
  author={Harvey B. Sarnat},
  journal={Brain & development},
  year={1987},
  volume={9 1},
  pages={43-7}
}
Acridine orange, a fluorochromic stain of nucleic acids, was used to study tissue sections of brain and spinal cord in 22 premature and full-term neonates who died within a week after severe fetal distress or birth asphyxia. Neurons suffering hypoxic injury may show either a centrifugal or a centripetal pattern of redistribution of cytoplasmic RNA. Severely involved cells show almost complete loss of orange RNA fluorescence, as well as decreased or condensed DNA fluorescence of nuclear… CONTINUE READING