Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein.

@article{Kikuchi2008HypoxiaIE,
  title={Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein.},
  author={Yutaka Kikuchi and Tomoshi Kakeya and Osamu Nakajima and Ayako Sakai and Kikuko Ikeda and Noritaka Yamaguchi and Takeshi Yamazaki and Ken-ichi Tanamoto and Haruo Matsuda and Jun-ichi Sawada and Kosuke Takatori},
  journal={The FEBS journal},
  year={2008},
  volume={275 11},
  pages={2965-76}
}
The human prion protein (PrP) is a glycoprotein with a glycosylphosphatidylinositol (GPI) anchor at its C-terminus. Here we report alternative splicing within exon 2 of the PrP gene (PRNP) in the human glioblastoma cell line T98G. The open reading frame of the alternatively spliced mRNA lacked the GPI anchor signal sequence and encoded a 230 amino acid polypeptide. Its product, GPI-anchorless PrP (GPI(-) PrPSV), was unglycosylated and soluble in non-ionic detergent, and was found in the… CONTINUE READING