Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner.

  title={Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner.},
  author={Lucia Bin{\'o} and Jiřina Proch{\'a}zkov{\'a} and Katarzyna Anna Radaszkiewicz and Jan Ku{\vc}era and Jana Kudov{\'a} and Jiř{\'i} Pachern{\'i}k and Luk{\'a}{\vs} Kubala},
The potentiation of the naturally limited regenerative capacity of the heart is dependent on an understanding of the mechanisms that are activated in response to pathological conditions such as hypoxia. Under these conditions, the expression of genes suggested to support cardiomyocyte survival and heart adaptation is triggered. Particularly important are changes in the expression of myosin heavy chain (MHC) isoforms. We propose here that alterations in the expression profiles of MHC genes are… Expand
HIF-1, Metabolism, and Diabetes in the Embryonic and Adult Heart
The function of HIF-1 in the heart throughout development into adulthood, as well as the deregulation of Hif-1 signaling in diabetes and its effects on the embryonic and adult heart are discussed. Expand
The influence of hypoxia on the prostate cancer proteome
Evaluated the impact of hypoxia on the proteome of the prostate to establish whether NO-NSAID treatment reverted the protein profiles back to their normoxic status and demonstrated well-defined, significantly regulated/differentially expressed proteins primarily involved with structural and binding processes. Expand


Enhancement of glycolysis in cardiomyocytes elevates endothelin-1 expression through the transcriptional factor hypoxia-inducible factor-1 alpha.
The findings indicate that the intrinsic energy metabolic system in cultured cardiomyocytes in vitro is predominantly glycolysis, and that the gene expression of cardiac ET-1 parallels the state of the Glycolytic system. Expand
Hypoxia-induced switches of myosin heavy chain iso-gene expression in rat heart.
It is concluded that hypoxia per se induces a pattern of isoform gene expression associated with early cardiac development, both in vivo and in vitro. Expand
Inhibitory PAS domain protein is a negative regulator of hypoxia-inducible gene expression
An inhibitory PAS (Per/Arnt/Sim) domain protein, IPAS, which is a basic helix-loop-helix (bHLH)/PAS protein structurally related to HIFs, is described, which demonstrates dominant negative regulation of HIF-mediated control of gene expression. Expand
Activation of Hypoxia-inducible Transcription Factor Depends Primarily upon Redox-sensitive Stabilization of Its α Subunit*
Results suggest that an intact redox-dependent signaling pathway is required for destablization of the HIF-1α protein, which was rapidly and drastically decreased in vivo following an abrupt increase to normal oxygen tension. Expand
The stabilization of hypoxia inducible factor modulates differentiation status and inhibits the proliferation of mouse embryonic stem cells.
The short term stabilization of HIF mediated by the PHD inhibitors JNJ and DMOG and hypoxia did not prevent the spontaneous loss of pluripotency markers in mESC, however, it significantly downregulated the proliferation of these cells. Expand
Hypoxia differentially regulates muscle oxidative fiber type and metabolism in a HIF-1α-dependent manner.
This study shows that hypoxia differentially regulates contractile and metabolic components of muscle oxidative phenotype in a HIF-1α-dependent manner. Expand
Role of HIF-1α in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis
It is shown that hypoxia and hypoglycaemia reduce proliferation and increase apoptosis in wild-type (Hif-1α+/+) embryonic stem (ES) cells, but not in ES cells with inactivated HIF-1 α genes (HIF- 1α−/−), suggesting that there are at least two different adaptive responses to being deprived of oxygen and nutrients. Expand
Purification and Characterization of Hypoxia-inducible Factor 1 (*)
  • Guang L. Wang, G. Semenza
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 1995
The biochemical purification of HIF-1 from Epo-producing Hep3B cells and non-Epo-producing HeLa S3 cells concludes that in both cobalt chloride-treated HeLa cells and hypoxic Hep3 B cells HIF -1 is composed of two different subunits: 120-kDa Hif-1α and 91-94-k da HIF,1β. Expand
Early expression of angiogenesis factors in acute myocardial ischemia and infarction.
An increase in the level of HIF-1alpha is an early response to myocardial ischemia or infarction, which defines, at a molecular level, one of the first adaptations of human myocardium to a deprivation of blood. Expand
Hypoxia Inducible Factor-1 Activation by Prolyl 4-Hydroxylase-2 Gene Silencing Attenuates Myocardial Ischemia Reperfusion Injury
In vitro and in vivo, PHD2 silencing using a siRNA strategy produces transcriptionally active HIF-1, which attenuates reperfusion injury via an iNOS-dependent pathway and stabilization in normoxia murine microvascular endothelial cells. Expand