Hypoxia/Notch signaling in primary culture of rat lymphatic endothelial cells.

@article{Ota2007HypoxiaNotchSI,
  title={Hypoxia/Notch signaling in primary culture of rat lymphatic endothelial cells.},
  author={Hideki Ota and Ken-ichi Katsube and Jyun-ichi Ogawa and Masaki Yanagishita},
  journal={FEBS letters},
  year={2007},
  volume={581 27},
  pages={
          5220-6
        }
}
We have developed an improved intralumenal digestion method to get a long-term primary culture of rat lymphatic endothelial cells (rLECs) that maintained their original phenotypes. rLECs in vitro under hypoxia retained their original lymphatic properties observed in the thoracic duct. Blocking Notch signal with a gamma-secretase inhibitor and transfection of a cDNA expressing a dominant negative form of Delta1 indicated that Notch signal downregulated VEGFR-2 under hypoxia and inhibited cell… CONTINUE READING

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Lymphangiogenesis: in vitro and in vivo models.

  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2010
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