Hypotonic hemolysis of human red blood cells: a two-phase process

  title={Hypotonic hemolysis of human red blood cells: a two-phase process},
  author={Jack T. Saari and James S. Beck},
  journal={The Journal of Membrane Biology},
Previous use of hemolysis time measurement to determine permeability coefficients for the red blood cell membrane rested on the assumption that cells swelling in a hypotonic medium hemolyzed immediately on reaching critical volume. By preswelling red cells to various volumes prior to immersion in hemolytic solutions we extrapolate to the hemolysis time of red cells immersed at critical volume and thereby find a significant period of time during which the cells apparently remain in a spherical… CONTINUE READING


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