Hypotaurine aminotransferase assay: [2-3H]hypotaurine.

  title={Hypotaurine aminotransferase assay: [2-3H]hypotaurine.},
  author={J. Fellman},
  journal={Methods in enzymology},
  • J. Fellman
  • Published 1987
  • Biology, Medicine
  • Methods in enzymology
Publisher Summary This chapter discusses the assay of hypotaurine aminotransferase as is the synthesis of the substrate of the enzyme, [2- 3 H]hypotaurine. Hypotaurine aminotransferase catalyzes the α-ketoglutarate-dependent transamination of hypotaurine to form sulfinoacetaldehyde, an unstable intermediate, which rapidly degenerates to acetaldehyde and SO 2 . The rate of the enzyme reaction can be monitored by determining the rate of formation of glutamate, SO 2 , or acetaldehyde, the rate is… Expand


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