Hypotaurine aminotransferase assay: [2-3H]hypotaurine.

@article{Fellman1987HypotaurineAA,
  title={Hypotaurine aminotransferase assay: [2-3H]hypotaurine.},
  author={J. Fellman},
  journal={Methods in enzymology},
  year={1987},
  volume={143},
  pages={
          183-5
        }
}
  • J. Fellman
  • Published 1987
  • Biology, Medicine
  • Methods in enzymology
Publisher Summary This chapter discusses the assay of hypotaurine aminotransferase as is the synthesis of the substrate of the enzyme, [2- 3 H]hypotaurine. Hypotaurine aminotransferase catalyzes the α-ketoglutarate-dependent transamination of hypotaurine to form sulfinoacetaldehyde, an unstable intermediate, which rapidly degenerates to acetaldehyde and SO 2 . The rate of the enzyme reaction can be monitored by determining the rate of formation of glutamate, SO 2 , or acetaldehyde, the rate is… Expand

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TLDR
Differences in sedimentation constant, and enzyme kinetic and the association of the second peak with aspartate aminotransferase led us to discuss the possible existence of molecular complexes. Expand
Irreversible inactivation of pig brain γ-aminobutyric acid-α-ketoglutarate transaminase by 4-amino-5-halopentanoic acids
Abstract γ-Aminobutyric acid-α-ketoglutarate transaminase from pig brain is irreversibly inactivated by 4-amino-5-halopentanoic acids. Protection from inactivation by the natural substrates, the pHExpand
Mammalian hypotaurine aminotransferase: isethionate is not a product.
Abstract We report that mammalian tissues posses hypotaurine (2-aminoethane- sulfinate) aminotransferase activity. One product of transamination, sulfinoacetaldehyde could theoretically undergoExpand
The synthesis of [2‐3H2] taurine and [2‐3H2] hypotarine
The synthesis of [2-3H2]-2-aminoethanesulfonate [2-3H]-taurine by the reduction of cyanomethanesulfonic acid with tritium gas is described. The conversion of [2-3H]-taurine and its 14C and 35SExpand