Hyposialylation of high‐molecular‐weight membrane glycoproteins parallels the loss of metastatic potential in wheat‐germ agglutinin‐resistant friend leukemia cells

@article{Benedetto1989HyposialylationOH,
  title={Hyposialylation of high‐molecular‐weight membrane glycoproteins parallels the loss of metastatic potential in wheat‐germ agglutinin‐resistant friend leukemia cells},
  author={Arrigo Benedetto and Giuliano Elia and A. Sala and F. Belardeli},
  journal={International Journal of Cancer},
  year={1989},
  volume={43}
}
From the highly metastatic in vivo‐passaged Friend leukemia cells (FLC), WGA‐resistant (WR) tumor cell variants were selected. These WR FLC had lost their capacity to metastasize when injected i.v. or s.c. into DBA/2 mice. We have characterized the plasma membrane glycoproteins of the different FLC types by: (i) metabolic labelling with (3H)‐galactose; (ii) surface labelling with galactose oxidase‐borohydride; (iii) direct binding of (125l)‐lectins on glycoproteins separated by SDS‐PAGE. The… 
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