Hypocretin‐1 causes G protein activation and increases ACh release in rat pons

  title={Hypocretin‐1 causes G protein activation and increases ACh release in rat pons},
  author={René Bernard and Ralph Lydic and Helen A. Baghdoyan},
  journal={European Journal of Neuroscience},
The effects of the arousal‐promoting peptide hypocretin on brain stem G protein activation and ACh release were examined using 16 adult Sprague‐Dawley rats. In vitro[35S]GTPγS autoradiography was used to test the hypothesis that hypocretin‐1‐stimulated G protein activation is concentration‐dependent and blocked by the hypocretin receptor antagonist SB‐334867. Activated G proteins were quantified in dorsal raphe nucleus (DR), locus coeruleus (LC) and pontine reticular nucleus oral part (PnO) and… 

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