Hypocotyl-based Agrobacterium-mediated transformation of soybean (Glycine max) and application for RNA interference

  title={Hypocotyl-based Agrobacterium-mediated transformation of soybean (Glycine max) and application for RNA interference},
  author={Geliang Wang and Yinong Xu},
  journal={Plant Cell Reports},
An efficient system of gene transformation is necessary for soybean [Glycine max (L.) Merrill] functional genomics and gene modification by using RNA interference (RNAi) technology. To establish such system, we improved the conditions of tissue culture and transformation for increasing the frequency of adventitious shoots and decreasing the browning and necrosis of hypocotyls. Adding N6-benzylaminopurine (BAP) and silver nitrate in culture medium enhanced the shoot formation on hypocotyls. BAP… CONTINUE READING
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