Hypobromous acid and bromamine production by neutrophils and modulation by superoxide.

  title={Hypobromous acid and bromamine production by neutrophils and modulation by superoxide.},
  author={Anna L. P. Chapman and Ojia Skaff and Revathy Senthilmohan and Anthony J. Kettle and Michael Jonathan Davies},
  journal={The Biochemical journal},
  volume={417 3},
MPO (myeloperoxidase) catalyses the oxidation of chloride, bromide and thiocyanate to their respective hypohalous acids. We have investigated the generation of HOBr by human neutrophils in the presence of physiological concentrations of chloride and bromide. HOBr was trapped with taurine and detected by monitoring the bromination of 4-HPAA (4-hydroxyphenylacetic acid). With 100 microM bromide and 140 mM chloride, neutrophils generated HOBr and it accounted for approx. 13% of the hypohalous… 

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