• Biology, Medicine
  • Published in Journal of cell science 1974

Hypertonicity and the arrest of mammalian cells in metaphase: A synchrony technique for HeLa cells.

@article{Wheatley1974HypertonicityAT,
  title={Hypertonicity and the arrest of mammalian cells in metaphase: A synchrony technique for HeLa cells.},
  author={Denys N Wheatley},
  journal={Journal of cell science},
  year={1974},
  volume={15 1},
  pages={
          221-37
        }
}
Raising the strength of culture medium from 149 or 165 mM by the addition of concentrated saline solutions to about 225-235 mM arrests HeLa cells for 3 h in metaphase without preventing the entry of G2 cells into mitosis. Apart from an initial period of 30 min, metaphases have well developed equatorial plates and chromosomes are discrete from one another. By employing a standardized continuous wash procedure for detachment of dividing cells during exposure of HeLa monolayers to hypertonicity… CONTINUE READING

References

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