Hyperspectral and differential CARS microscopy for quantitative chemical imaging in human adipocytes.

@article{Napoli2014HyperspectralAD,
  title={Hyperspectral and differential CARS microscopy for quantitative chemical imaging in human adipocytes.},
  author={Claudia Di Napoli and Iestyn Pope and Francesco Masia and P Watson and Wolfgang Langbein and Paola Borri},
  journal={Biomedical optics express},
  year={2014},
  volume={5 5},
  pages={1378-90}
}
In this work, we demonstrate the applicability of coherent anti-Stokes Raman scattering (CARS) micro-spectroscopy for quantitative chemical imaging of saturated and unsaturated lipids in human stem-cell derived adipocytes. We compare dual-frequency/differential CARS (D-CARS), which enables rapid imaging and simple data analysis, with broadband hyperspectral CARS microscopy analyzed using an unsupervised phase-retrieval and factorization method recently developed by us for quantitative chemical… CONTINUE READING

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