Hyperproduction of chitinase influences crystal toxin synthesis and sporulation of Bacillus thuringiensis

@article{BarbozaCorona2009HyperproductionOC,
  title={Hyperproduction of chitinase influences crystal toxin synthesis and sporulation of Bacillus thuringiensis},
  author={Jos{\'e} E Barboza-Corona and Tom{\'a}s Ortiz-Rodr{\'i}guez and Norma M. de la Fuente-Salcido and Dennis K Bideshi and Jorge E. Ibarra and Rub{\'e}n Salcedo-Hern{\'a}ndez},
  journal={Antonie van Leeuwenhoek},
  year={2009},
  volume={96},
  pages={31-42}
}
Bacillus thuringiensis HD-73 was transformed with the endochitinase gene chiA74 under the control of a strong promoter (pcytA) and a 5′ mRNA stabilizing (STAB-SD) sequence (HD-73-pEBchiA74). Expression levels were compared with those observed from the wild type strain (HD-73) and the recombinant HD-73 strain expressing chiA74 under the control of its native promoter (HD-73-pEHchiA74). The chitinolytic activity of HD-73-pEBchiA74 was markedly elevated, being ~58- and 362-fold higher than… CONTINUE READING

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