Hyperpolarized 13C allows a direct measure of flux through a single enzyme-catalyzed step by NMR.

@article{Merritt2007Hyperpolarized1A,
  title={Hyperpolarized 13C allows a direct measure of flux through a single enzyme-catalyzed step by NMR.},
  author={Matthew E Merritt and Crystal E Harrison and Charles J Storey and F. M. H. Jeffrey and A Dean Sherry and Craig Malloy},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2007},
  volume={104 50},
  pages={19773-7}
}
(13)C NMR is a powerful tool for monitoring metabolic fluxes in vivo. The recent availability of automated dynamic nuclear polarization equipment for hyperpolarizing (13)C nuclei now offers the potential to measure metabolic fluxes through select enzyme-catalyzed steps with substantially improved sensitivity. Here, we investigated the metabolism of hyperpolarized [1-(13)C(1)]pyruvate in a widely used model for physiology and pharmacology, the perfused rat heart. Dissolved (13)CO(2), the… CONTINUE READING

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