Hyperploid cells in the limb buds of rats and 5-azacytidine-induced digital deformities.


To clarify the mechanism underlying the digital teratogenesis of 5-azacytidine (5-AC), the DNA contents of mesenchymal cells were examined in the limb buds of the rat by microspectrophotometry. 5-AC induced hyperploid cells: 2.4% at 18 h after injection and 3.2% at 24 h. No hyperploid cells were noted 30 h after the treatment. Because posttreatment caffeine… (More)