Hyperosmotic induction of mitogen-activated protein kinase scaffolding.

  title={Hyperosmotic induction of mitogen-activated protein kinase scaffolding.},
  author={Thomas L. Hilder and Michael H. Malone and Gary Leon Johnson},
  journal={Methods in enzymology},
Eukaryotic cells respond to hyperosmotic conditions by expunging water from the cell, leading to cell shrinkage. This is counteracted by adaptive responses that restore cell volume and strengthen the cytoskeletal architecture. In the budding yeast Saccharomyces cerevisiae, this response is mediated primarily by the mitogen-activated protein kinase (MAPK) cascade CDC42-STE50-STE11-Pbs2-Hog1. In mammalian cells, MAPK scaffold proteins facilitate the efficiency of signaling within the cascade by… CONTINUE READING