Hypermethylation of the PTEN gene in ovarian cancer cell lines.

  title={Hypermethylation of the PTEN gene in ovarian cancer cell lines.},
  author={Thomas Sch{\"o}ndorf and Matthias P. A. Ebert and Juliane Hoffmann and Martina Becker and Natasha Moser and Saziye Pur and U J G{\"o}hring and Maria-Paz Weisshaar},
  journal={Cancer letters},
  volume={207 2},
This study was performed to investigate the hypermethylation status of the PTEN gene in ovarian cancer. To this end, we incubated eight ovarian cancer cell lines with the demethylating agent 5-aza-2' deoxycytidine in three different concentrations for 5 days. Subsequently, the PTEN expression was quantified by both real time RT-PCR and quantitative western analyses. PTEN mRNA varied considerably in response to demethylation whereas PTEN protein concentrations remained constant in all cell lines… CONTINUE READING

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