Hyper-production and Characterization of the ι-Carrageenase Useful for ι-Carrageenan Oligosaccharide Production from a Deep-sea Bacterium, Microbulbifer thermotolerans JAMB-A94T, and Insight into the Unusual Catalytic Mechanism

@article{Hatada2010HyperproductionAC,
  title={Hyper-production and Characterization of the ι-Carrageenase Useful for ι-Carrageenan Oligosaccharide Production from a Deep-sea Bacterium, Microbulbifer thermotolerans JAMB-A94T, and Insight into the Unusual Catalytic Mechanism},
  author={Yuji Hatada and Masahiro Mizuno and Zhijun Li and Yukari Ohta},
  journal={Marine Biotechnology},
  year={2010},
  volume={13},
  pages={411-422}
}
A gene of unknown function from the genome of the agar-degrading deep-sea bacterium Microbulbifer thermotolerans JAMB-A94T was functionally identified as a ι-carrageenase gene. This gene, designated as cgiA, is located together with two β-agarase genes, agaA and agaO in a cluster. The cgiA gene product is 569 amino acids and shares 29% identity over 185 amino acids with the ι-carrageenase from Zobellia galactanivorans Dsij DSM 12802. Recombinant, cgiA-encoded ι-carrageenase (55 kDa) was hyper… CONTINUE READING

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Mechanism ofmild acid hydrolysis of galactan polysaccharides with highly ordered disaccharide repeats leading to a complete series of exclusively odd - numbered oligosaccharides

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  • FEBS J
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1 Excerpt

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