Hyper-inducible expression system for streptomycetes.


Streptomycetes produce useful enzymes and a wide variety of secondary metabolites with potent biological activities (e.g., antibiotics, immunosuppressors, pesticides, etc.). Despite their importance in the pharmaceutical and agrochemical fields, there have been no reports for practical expression systems in streptomycetes. Here, we developed a "P(nitA)-NitR" system for regulatory gene expression in streptomycetes based on the expression mechanism of Rhodococcus rhodochrous J1 nitrilase, which is highly induced by an inexpensive and safe inducer, epsilon-caprolactam. Heterologous protein expression experiments demonstrated that the system allowed suppressed basal expression and hyper-inducible expression, yielding target protein levels of as high as approximately 40% of all soluble protein. Furthermore, the system functioned in important streptomycete strains. Thus, the P(nitA)-NitR system should be a powerful tool for improving the productivity of various useful products in streptomycetes.

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@article{Herai2004HyperinducibleES, title={Hyper-inducible expression system for streptomycetes.}, author={Sachio Herai and Yoshiteru Hashimoto and Hiroki Higashibata and Hideaki Maseda and Haruo Ikeda and Satoshi O̅mura and Michihiko Kobayashi}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={2004}, volume={101 39}, pages={14031-5} }