Hydroxyurea ablation of mushroom bodies in Drosophila.

  title={Hydroxyurea ablation of mushroom bodies in Drosophila.},
  author={Sean T Sweeney and Alicia Hidalgo and J. Steven de Belle and Haig Keshishian},
  journal={Cold Spring Harbor protocols},
  volume={2012 2},
Chemical ablation is an effective tool for studying nervous system development and function in Drosophila. Hydroxyurea (HU) inhibits ribonucleotide reductase, blocking DNA synthesis, and killing dividing cells. The specificity of HU ablation is thus dependent on developmental events. In this respect, HU is useful in determining temporal patterns of neuroblast proliferation and the origins of neuronal elements in flies and other insects. In Drosophila, an especially fortuitous time window occurs… CONTINUE READING

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