Hydroxyl radical " footprinting " : High-resolution information about DNA-protein contacts and application to X repressor and Cro protein

@inproceedings{TulliusHydroxylR,
  title={Hydroxyl radical " footprinting " : High-resolution information about DNA-protein contacts and application to X repressor and Cro protein},
  author={Thomas D. Tullius and BETH A. DOMBROSKI}
}
A method has been developed for making "footprints" of proteins bound to DNA. The hydroxyl radical, generated by reduction of hydrogen peroxide by iron(ll), is the reagent used to cut the DNA. Hydroxyl radical breaks the backbone of DNA with almost no sequence dependence, so all backbone positions may be monitored for contact with protein. In addition to defining the DNA sequence in contact with the protein, hydroxyl radical footprints embody structural information about the DNA-protein complex… CONTINUE READING