Hydrostatic pressure induces expression of interleukin 6 and tumour necrosis factor alpha mRNAs in a chondrocyte-like cell line.

@article{Takahashi1998HydrostaticPI,
  title={Hydrostatic pressure induces expression of interleukin 6 and tumour necrosis factor alpha mRNAs in a chondrocyte-like cell line.},
  author={Kazushige Takahashi and Toshikazu Kubo and Yuji Arai and Isao Kitajima and Masaharu Takigawa and Jiro Imanishi and Yasusuke Hirasawa},
  journal={Annals of the rheumatic diseases},
  year={1998},
  volume={57 4},
  pages={231-6}
}
OBJECTIVES To clarify the effect of pressure on the expressions of proteoglycan core protein and metabolism related cytokines in a chondrocyte-like cell line, HCS-2/8. METHODS HCS-2/8 cells were exposed to 1, 5, 10, or 50 MPa of hydrostatic pressure (HP) for two hours, and mRNA expressions of interleukin 6 (IL6) and tumour necrosis factor alpha (TNFalpha) were examined by using reverse transcription-polymerase chain reaction (RT-PCR) method with specific primer sets; and mRNA of proteoglycan… CONTINUE READING