Hydrophobic interaction chromatography correctly refolding proteins assisted by glycerol and urea gradients.

@article{Li2004HydrophobicIC,
  title={Hydrophobic interaction chromatography correctly refolding proteins assisted by glycerol and urea gradients.},
  author={Jing-jing Li and Yong-dong Liu and Fang-wei Wang and Guanghui Ma and Zhi-guo Su},
  journal={Journal of chromatography. A},
  year={2004},
  volume={1061 2},
  pages={193-9}
}
Chromatographic columns packed with commercially available hydrophobic interaction chromatography (HIC) media were found to be able to suppress aggregation and nevertheless had a tendency to promote the structural misfolding resulting in higher soluble protein recovery and lower specific activity than that by dilution when they were used to refold lysozyme, a model protein. Moreover, this misfolding effect was exacerbated with increasing hydrophobicity of media. A novel strategy involving the… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 13 references

Protein Expr

K. Tsumoto, D. Ejima, I. Kumagai, T. Arakawa
Purif. 28 • 2003

Int

F. Meng, Y. Park, H. Zhou
J. Biochem. Cell Biol. 33 • 2001
View 1 Excerpt

J

Z. Gu, Z. Su, J. C. Janson
Chromatogr. A 918 • 2001

Trends Cell

R. R. Kopito
Biol. 10 • 2000
View 1 Excerpt

Triends Biochem

S. E. Radford
Sci. 25 • 2000
View 1 Excerpt

Protein Sci

S. K. Kulkarni, A. E. Ashcroft, +3 authors S. E. Radford
8 • 1999
View 1 Excerpt

FEBS Lett

H. Rogl, K. Kosemund, W. Kühlbrandt, I. Collinson
432 • 1998

Proc

R. V. Rariy
Natl. Acad. Sci. U.S.A. 94 • 1997
View 1 Excerpt

Faseb J

R. Rudolph, H. Lille
10 • 1996
View 1 Excerpt

P

B. Fischer, L. Sumner
Goodenough, Arzneimittelforschung 42 • 1992
View 1 Excerpt

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