Hydrophobic Substitution of Surface Residues Affects Lipase Stability in Organic Solvents

@article{Shokri2013HydrophobicSO,
  title={Hydrophobic Substitution of Surface Residues Affects Lipase Stability in Organic Solvents},
  author={Maryam Monsef Shokri and Shahin Ahmadian and Neda Akbari and Khosro Khajeh},
  journal={Molecular Biotechnology},
  year={2013},
  volume={56},
  pages={360-368}
}
A novel lipase has been recently isolated from a local Pseudomonas sp. (GQ243724). In the present study, we have tried to increase the organic solvent stability of this lipase using site-directed mutagenesis. Eight variants N219L, N219I, N219P, N219A, N219R, N219D, S251L, and S251K were designed to change the surface hydrophobicity of this enzyme with respect to the wild-type. Among these variants, the stability of N219L and N219I significantly increased in the presence of all tested organic… CONTINUE READING

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