Hydrophobic Fluorescent Probes Introduce Artifacts into Single Molecule Tracking Experiments Due to Non-Specific Binding

@inproceedings{ZanettiDomingues2013HydrophobicFP,
  title={Hydrophobic Fluorescent Probes Introduce Artifacts into Single Molecule Tracking Experiments Due to Non-Specific Binding},
  author={Laura C. Zanetti-Domingues and Christopher J. Tynan and Daniel J. Rolfe and David T. Clarke and Marisa L. Martin-Fernandez},
  booktitle={PloS one},
  year={2013}
}
Single-molecule techniques are powerful tools to investigate the structure and dynamics of macromolecular complexes; however, data quality can suffer because of weak specific signal, background noise and dye bleaching and blinking. It is less well-known, but equally important, that non-specific binding of probe to substrates results in a large number of… CONTINUE READING