Hydrolytic cleavage of nascent RNA in RNA polymerase III ternary transcription complexes.

@article{Whitehall1994HydrolyticCO,
  title={Hydrolytic cleavage of nascent RNA in RNA polymerase III ternary transcription complexes.},
  author={Simon K. Whitehall and Carolyne Bardeleben and George A. Kassavetis},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 3},
  pages={2299-306}
}
Highly purified yeast RNA polymerase III ternary complexes were found to possess a hydrolytic chain retracting activity that cleaves nascent RNA from its 3'-OH end. Most of the shortened transcripts were capable of resuming RNA chain elongation, indicating that they remain stably associated with the enzyme-DNA complex. Analysis of the products of cleavage indicated that retraction primarily occurred in dinucleotide increments, but that mononucleotides were also excised at lower frequency. The… CONTINUE READING