Hydrolytic activity in baker's yeast limits the yield of asymmetric 3-oxo ester reduction.

@article{ChinJoe2000HydrolyticAI,
  title={Hydrolytic activity in baker's yeast limits the yield of asymmetric 3-oxo ester reduction.},
  author={I Chin-Joe and P M Nelisse and Adrie J. J. Straathof and Jaap A. Jongejan and Jack T. Pronk and Joseph J. Heijnen},
  journal={Biotechnology and bioengineering},
  year={2000},
  volume={69 4},
  pages={370-6}
}
Microbial reductions of ketones hold great potential for the production of enantiopure alcohols, as long as highly selective redox enzymes are not interfered with by competing activities. During reduction of ethyl 3-oxobutanoate by baker's yeast (Saccharomyces cerevisiae) to ethyl (S)-3-hydroxybutanoate, a high enantiomeric excess (> 99%) can be obtained. However, reported yields do not exceed 50-70%. In this article, three main causes are shown to be responsible for these low to moderate… CONTINUE READING