Hydrolysis reaction analysis of L-alpha-distearoylphosphatidylcholine monolayer catalyzed by phospholipase A2 with polarization-modulated infrared reflection absorption spectroscopy.

@article{Wang2005HydrolysisRA,
  title={Hydrolysis reaction analysis of L-alpha-distearoylphosphatidylcholine monolayer catalyzed by phospholipase A2 with polarization-modulated infrared reflection absorption spectroscopy.},
  author={Xiaoli Wang and Suping Zheng and Qiang He and Gerald Brezesinski and Helmuth M{\"o}hwald and Junbai Li},
  journal={Langmuir : the ACS journal of surfaces and colloids},
  year={2005},
  volume={21 3},
  pages={
          1051-4
        }
}
The hydrolysis reaction of L-alpha-distearoylphosphatidylcholine (DSPC) monolayers catalyzed by phospholipase A2 (PLA(2)) has been studied using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) with film balance measurements. The PM-IRRAS analysis provides quantitative information about the reaction efficiency at different surface pressures. It was found that the reaction efficiency of L-DSPC monolayer hydrolysis catalyzed by PLA(2) decreased with increasing surface… CONTINUE READING

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