Hydrolysis of triacetin catalyzed by immobilized lipases: effect of the immobilization protocol and experimental conditions on diacetin yield.

@article{Hernndez2011HydrolysisOT,
  title={Hydrolysis of triacetin catalyzed by immobilized lipases: effect of the immobilization protocol and experimental conditions on diacetin yield.},
  author={Karel Morlans Hern{\`a}ndez and Eduardo Garc{\'i}a-Verdugo and Ra{\'u}l Porcar and Roberto Fernandez-Lafuente},
  journal={Enzyme and microbial technology},
  year={2011},
  volume={48 6-7},
  pages={510-7}
}
The effect of the immobilization protocol and some experimental conditions (pH value and presence of acetonitrile) on the regioselective hydrolysis of triacetin to diacetin catalyzed by lipases has been studied. Lipase B from Candida antarctica (CALB) and lipase from Rhizomucor miehei (RML) were immobilized on Sepabeads (commercial available macroporous acrylic supports) activated with glutaraldehyde (covalent immobilization) or octadecyl groups (adsorption via interfacial activation). All the… CONTINUE READING